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ANTIBODIES OF ESCHERICHIA COLI UROPATHOGENIC ON AN EXPERIMENTAL MODEL

Lubetkin Alberto. Garimaldi Jorge. Di Santo Lucrecia. Brarda Oscar. Gonzalez Q., Hector., Bertone Patricia. Zubeldia Daniel.

FUMCER. Alvear 1339. (5800) Rio Cuarto. Cordoba. Argentina.

Introduction: a) The Urinary Tract Infection (U.T.I.) is a frequent cause of morbidity in children, adolescents and adults. b) The role of Immunity in “Recurrent Urinary Tract Infections” (R.U.T.I.), is unsettled and polemic. c) In the patients with R.U.T.I., especially those with acute infection and more than the half of those with prolonged infection, the most frequent bacteria are Escherichia coli. Objectives: The purpuse is to evaluate the antigenetic response of an experimental model, as an answer to a strein of E. coli. Methods: Animals: Four groups each one with four rabbits. (Race: New Zealand) weigh 3.5 kg. average. They were named group 1,2,3 and 4. Immunization: It was used subcutaneous inoculation (1ml) of Pseudomona aeruginosa hapten in group 1. It was used P. Fimbriated Eschdomona coli DS17 (Sweedish) in group 2. It was used E. coli DS17 hapten in group 3. Group 4 was not immunised, and was used as control. Bacterial Inoculation: After 30 days 1ml of bacteria (10,7 E. coli DS 17) was inoculated into the bladder through a urethral catheter. Immunologic studies: Determination of lymphocytic activity to bacteraemic antigens and to non specific antigens (phytohemagglutinin M) before and after immunization: Test of bladder lymphocytic transformation. Determination of delayed hypersensitivity: cutaneous test. Other procedures: Clinical Evaluation Laboratory: Hemogram. Creatinine. Urine: Microscopic urinalysis and enzymatic procedures of screeening. Hemocytometer WB cells counts, urine and kidney culture. Pathological Evaluation: autopsy of bladder, ureters and kidneys using Electronic and Optical microscopy. Preliminary results: Each rabbit in Group 4, were infected. The urine remained with bacteriuria during a long period of time. Only one of them remained infected until it was sacrificed. In Group 1: at 7^th days, having been inoculated, rabbit No. 3 was infected. The others cured spontaneously. In Group 2: No. 5 and 7 were infected, and they also cured spontaneously. In Group 3: at 5^th days, No. 11 was infected and the infection disappeared spontaneously, as well. The kind of induced antibodies were evaluated. Optical and electronic Microscopy evaluated the histological alterations of the induced damages. Acknowledgment we would like to thank Prof. J. Winberg of the University of Stockholm for the help with the study.