0025
THE ACTIVITY
OF GLUCOSE-6-PHOSPHATASE AND THE STRUCTURE CHANGES OF MITOCHONDRIA IN THE
HEARTS OF SIRS RATS LIU Chunfeng, LI Wei, YUAN Zhuang, et al.
Department of Pediatrics, The 2nd
Clinical Hospital, China Medical
University, Shenyang 110003 Objective:To recognize
the metabolic changes of energy in SIRS and prevent SIRS from developing
into MODS, we searched changes of the activity of glucose-6-phosphatase and the cellular structure in the hearts of SIRS rats. Methods:(1)
High-dose endotoxin (LPS) induced the model of SIRS, then viewed the rats
whether the rectal temperature, heart rate, breathing rate, TNF-a in blood,
the amount of leukocyte and its classification had some alternations. (2)
Searched the activity of glucose-6-phosphatase in the hearts of SIRS rats
and analyzed with image analyzing machine. (3) Measured the blood
concentration of glucose in LPS groups before and after the injection of
LPS. (4) Viewed the pathological changes in SIRS rats with electron
microscope. Results:(1) 1 hour
after the LPS injection, the activity of glucose-6-phosphatase increased while 3 hours
after the injection decreased (P<0.05). (2) 1 hour after the LPS injection, the
concentration of glucose in the blood increased and 3 hours after
the injection decreased (P<0.05). (3) Under the electron microscope, we saw
the mitochondria in myocardium cells of SIRS rats increased in quantity and
had structure disorders. The myocardial fibers were edematous. Conclusions:The activity
of glucose-6-phosphatase in the hearts of SIRS rats changed and the glucose level in the
blood altered accordingly, in the
meanwhile, dysfunction and structure disorder appeared in myocardium mitochondria.