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THE ACTIVITY OF GLUCOSE-6-PHOSPHATASE AND THE STRUCTURE CHANGES OF MITOCHONDRIA IN THE HEARTS OF SIRS RATS

LIU Chunfeng, LI Wei, YUAN Zhuang, et al. Department of Pediatrics, The 2nd Clinical Hospital, China Medical University, Shenyang 110003

 

ObjectiveTo recognize the metabolic changes of energy in SIRS and prevent SIRS from developing into MODS, we searched changes of the activity of glucose-6-phosphatase and the cellular structure in the hearts of SIRS rats.

Methods(1) High-dose endotoxin (LPS) induced the model of SIRS, then viewed the rats whether the rectal temperature, heart rate, breathing rate, TNF-a in blood, the amount of leukocyte and its classification had some alternations. (2) Searched the activity of glucose-6-phosphatase in the hearts of SIRS rats and analyzed with image analyzing machine. (3) Measured the blood concentration of glucose in LPS groups before and after the injection of LPS. (4) Viewed the pathological changes in SIRS rats with electron microscope.

Results(1) 1 hour after the LPS injection, the activity of glucose-6-phosphatase increased while 3 hours after the injection decreased (P<0.05). (2) 1 hour after the LPS injection, the concentration of glucose in the blood increased and 3 hours after the injection decreased (P<0.05). (3) Under the electron microscope, we saw the mitochondria in myocardium cells of SIRS rats increased in quantity and had structure disorders. The myocardial fibers were edematous.

ConclusionsThe activity of glucose-6-phosphatase in the hearts of SIRS rats changed and the glucose level in the blood altered accordingly, in the meanwhile, dysfunction and structure disorder appeared in myocardium mitochondria.