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IRON DISTRIBUTION IN BRAIN WITH NEONATAL HYPOXIC-ISCHEMIC BRAIN DAMAGE

YU T¹, LIAO Q-K², LUO C-H², et al.

¹The Third People’s Hospital of Chengdu, Sichuan ,China

²West China University of Medical Sciences, Sichuan, China

Objective: To investigate relationship that mechanism of hypoxic-ischemic brain damage (HIBD) with iron distribution in brain tissues.

Methods: A 3’,3-diaminobezidine(DAB) intensification of the Prussian Blue stain was used to examine histochemically iron in the cerebral and cerebellar cortex and subcortex white matter from 6 autopsy neonates.

Results: 1、By imaging analyzer in DAB intensification of the Prussian Blue stain slides, area ratio in the cortex elevated more apparently than that in the subcortical white matter regardless of control or HIBD group, both P<0.01. Area ratio in the cortex and subcortex white matter of HIBD group was higher than that of control group, P<0.01 and P<0.05 separately. 2、Iron granules deposited markedly in the layer Ⅱ、Ⅲ、Ⅳ of the cerebral cortex and layerⅡof the cerebellar cortex. The predominant iron-positive cells were neurons and glial cells. Iron granules deposited in HIBD cortex were more than that in control cortex.

Conclusion: 1、Iron granules deposited in cortex tissue would increase when neonates suffered from HIBD. Region of iron deposited was just the same as that tissue susceptible to hypoxic ischemia in mature newborns. 2、The presence of iron within the cortex may be related to the elevated susceptibility of this region to oxidative damage in neonates.