0088
mRNA DIFFERENTIAL DISPLAY OF PROMYELOCYTIC LEUKEMIN
CELL LINES (HL-60): INDUCED & NON-INDUCED BY ALL-TRANS RETINOID ACID Gong F1, Liao QK2, Li FY2 1 Third Hospital of Chengdu, Chengdu,
China 2 Second Hospital of the West China
University of Medical Sciences, Chengdu, China Objective: To study the
differential display of promyelocytic leukemia cell lines induced and
non-induced by all-trans retinoid acid (ATRA). Methods: cDNA fragment was
obtained by reverse transcription-polymerse chain reaction (RT-PCR) with
total RNA extracted from promyelocytic leukemia cell lines. The amplified
cDNA fragments were confirmed on non-denaturing polyacrylamide gel. Results: With 24 primer
combinations,we amplified a total of 541 bands,the mean number of bands
obtained per lane was 23, and 36 bands were found with obvious difference. Conclusion: mRNA differential
display is effective to detect and characterize altered gene expressions in
promyelocytic leukemia cell lines induced and non-induced by ATRA.