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THE
EFFECT OF RICH-IRON AND IRON-DEPRIVED ON PROLIFERATION AND APPTOSIS OF
HL-60 CELLS
Liu Yufeng, Jia Guocun, Zeng Li,Wu Xueqiang, Sheng Guangyao
Department of
Pediatrics, First Affiliated Hospital of Henan Medical University,
Zhengzhou, China
Objective: To investigate
the effect of proliferation and apoptosis by iron, iron chelator and iron
or iron-chelator with chemotherapeutic agents on HL-60 cells.
Methods: HL-60
cells were cultured with different concentrations of iron (Ⅲ) chloride (FeCl3
), Iron-chelator-desferrioxamine (DFO), and cytosine arabinoside
(Ara-c ) or etoposide (VP-16). Proliferation on HL-60 cells was observed
by cell viability, apoptosis induced was investigated by cell morphology,
flow cytometry assay, DNA gel electrophoresis and the expression of C-myc
or Bcl-2 by immunocytochemistry methods.
Result: The
results showed that when HL-60 cells were incubated with different
concentrations of FeCl3 at 6h, 12h, 24h, 48h, the
proliferation of HL-60 was exuberant and APO% (apoptosis percentage) was
lower than that of control group (P<0.05) at each point, especially at
the concentration of 100μmol/L FeCl3. As the same time,
The numbers of bcl-2 expression positive cells were much higher than that
of control groups too (P<0.05). APO% of Ara-c or VP-16+ FeCl3
groups was lower than that of Ara-c and VP-16 at 6h, 12h, 24h (P<0.01).
When HL-60 cells were incubated with DFO, the proliferation of HL-60 was
decreased and APO% showed dose-time dependant was much higher than that
of control group (P<0.01). Apoptotic peak appeared at the
concentration of 100μmol/L, 150μmol/L DFO, meanwhile, the expression
of C-myc on HL-60 cells in DFO group was higher than that of control
group at 6h, 12h, 24h (P<0.05). APO% of DFO + Ara-c or VP-16 group at
6h, 12h, 24h was very higher than that of Ara-c or VP-16 (P<0.01). But
comparing FeCl3 + DFO + chemotherapeutic agents group with
chemotherapeutic agents group, APO% was no difference (P>0.05).
Conclusions:
Rich-iron could promote growth and inhibit apoptosis of HL-60 cells, and
iron-deprived could induced apoptosis of HL-60 cells. So, Iron-deprived
could be used to treat leukemia or other tumors by conjuncting with
anticancer agents. Meanwhile, according to the experimental results, we also
suggest that the caution needs to be raised in iron supplementation of
patients with tumor or people who have high risk of developing tumor, as
long as there are sufficient hemoglobin to maintain the survivial, blood
transfusion may not be necessary for patients with tumor.
Key words: iron;
HL-60 cell; apoptosis
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