0120

ASPIRIN INHIBITS THE APOPTOSIS AND NECROSIS INDUCED BY PEROXIDE IN HUMAN SMMC7721 HEPATOMA CELLS

He Z-X¹, Liao Q-K, Luo C-H, et al.

¹Department of Pediatrics, Second Affiliate Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou, China

Objective: To explore the effects of aspirin on hydrogen peroxide (H₂O₂) induced apoptosis and necrosis in human SMMC7721 hepatoma cells.

Methods: Using cytomorphology, DNA fragment gelelectrophoresis and flow cytometry, and lactate dehydrogenase (LDH) release rate, cell viability and cellular ferritin concentration were measured.

Results: 0.8mmol/L H₂O₂ caused apoptotic morphological characteristics in SMMC7721 cells, including aneuploid peak (AP) - apoptotic bodies occurrence by flow cytometry, and DNA ladder was observed by agarose gel electrophoresis.Whereas, high concentration of H₂O₂ (4mmol/L) treatment caused a significant necrosis in SMMC7721 cells. Then aspirin showed a marked cytoprotection toward SMMC7721 cells with inhibiting cellular apoptosis and necrosisfrom H₂O₂. 0.5mmol/L aspirin treated group reduced the rate of cellular apoptosis about 34%, LDH release rate reduced 40%, and cell viability increased 50 %. The cytoprotection from aspirin in SMMC7721 cells showed a dose dependent manner. When hepatoma cells were incubated with 5mmol/L aspirin could completely disappear the apoptosis and necrosis caused by H₂O₂ . The cytoprotection of aspirin was mimicked by apo-ferritin, and we found that aspirin induced significant increase ferritin expression in SMMC7721 cells，and it has a significant correlation with apoptosis and necrosis from H₂O₂.

Conclusion: Aspirin induced greatly increase on ferritin expression in SMMC7721 hepatoma cells for resistance to apoptosis and necrosis caused by H₂O₂.