EFFECTS OF IVIG ON TH1/TH2 OF CHILDREN WITH KD

Pi G-H¹, Yang X-Q², Wei Y-S¹

¹Affiliated Hospital, North Sichuan Medical College, Nanchong, China

²Children Hospital, Chongqing University of Medical Science, Chongqing, China

Objective: To investigate effects of IVIG on TH1/TH2 function during the acute phase in Kawasaki Disease (KD).

Methods: The levels of IL-4 and INF-γin the supernatants of cultured PBMC for 72 hours, or plasma IgE were measured by ELISA and CD4+CD30+ T cells of cultured PBMC for 72 hours were analyzed by FACS in the pre-treatment or the post-treatment (3~5days) of IVIG from 15 children with KD, respectively. Fifteen healthy children were served as normal controls.

Results: Expression rate of CD4+CD30+ T cells was markedly higher in the pre-treatment of IVIG during the acute phase of KD than in the post-treatment (t=7.02, p<0.01) and than controls (u=5.38, p<0.01). The IL-4 levels were significantly increased in the pre-treatment than in the post-treatment (t=9.65, p<0.01), and than controls (u=5.27, p<0.01). The INF-γlevels were not significantly different in the pre-treatment than in the post-treatment (t=0.78, p>0.05), but it was markedly lower than control(u=1.97, p<0.05). The IgE levels were significantly lower in the post-treatment than in the pretreatment (t=4.25, p<0.01), it was not significantly different than control (u=1.83, P>0.05).

Conclusion: TH1/TH2 function in the acute phase of KD was unbalanced. TH2 were increased. Imbalanced TH1/TH2 was improved and the enhanced TH2 function was rapidly reversed by IVIG.

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