0177

THE EFFECTS OF INTERLEUKIN-10 ON THE ENDOTHELIAL DAMAGE IN KAWASAKI DISEASE

Fu JR¹ , Li CR¹, Zhou YD² , Yang XQ²

¹ Children’s Hospital of Shenzhen, Shenzhen, China

² Children’s Hospital of Chongqing, Chongqing, China

Objective: To explore the effects of Interleukin-10 (IL-10) on the endothelial damage mediated by inflammatory cytokines in kawasaki diseae (KD).

Methods : Human umbilical vein endothelial cells were cultured in vitro and the production of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), IL-10 were measured by enzyme-linked immunosorbent assay (ELISA).The proportion of apoptotic cells in endothelial cells induced by the supernatants of peripheral blood mononuclear cells (PBMCs) was detected by AnnexinⅤ/PI double-staining though flow cytometry . Electrophoretic mobility shift assay (EMSA) was used to detect the activity of nuclear factor-κB (NF-κB).

Results : The level of TNF-α, IL-1β,IL-6 , IL-10 were markedly increased in KD patients and the proportion of apoptotic cells in endothelial cells induced by the cultured supernatants of PBMCs was markedly elevated (38.45±7.80%) compared to 2.87±0.76% in the control subjects .The apoptosis of endothelial cells induced by the cultured supernatants of PBMCs could be reversed to some degree by anti-TNF-α, IL-1β, IL-6 monoclonal antibody (McAb) , otherwise could be enhanced (58.62 ±14.55%) by anti-IL-10 McAb . The activity of NF-κB in the activated PBMCs in KD patients was distinctly increased and IL-10,could significantly inhibit the activity of NF-κB, could also reduce the production of TNF-α, IL-1β, IL-6 and the apoptosis of endothelial cells induced by the cultured supernatants of PBMCs in KD patients.

Conclusion : IL-10, which can inhibit the activity of NF-κB and the transcription of inflammatory cytokines such as TNF-α, IL-1β, IL-6 , act as an important protective factor in endothelial damage of KD.