An experimental study on content alteration of intracellular calcium ion in right ventricular myocytes during pneumonia

Chen Xinmin

Dong Fang Hospital ，Fuzhou /Fujian ，PROC

Objective：To study alteration in content of myocardial intracellular Ca²⁺ due to acute pneumonia under both quiescent and contracting conditions , there to get a good understanding of the possible mechanism of heart failure secondary to acute pneumonia.

Methods： Animal models of acute pneumonia were constructed by inoculating via trachea staphylococci aureus into 50-day old Wistar rats. Right ventricular myocytes were enzymatically isolated and display intracellular calcium ions was marked by fluo-3-Am.24 or 72 hours after inoculation fluorescence intensities of isolated myocytes were compared with those of controls under under both quiescent and contracting conditions.

Results：When 24 hours after inoculation, little change occurs to the content of intracellular Ca²⁺ of quiescent myocytes and 2.5 minutes of exposure duration extended to 5 or 7.5 or 10 minutes, no significant difference could still be found. When 72 hours after inoculation, intracellular calcium ions content was significantly higher under quiescent conditions, but lower during lower during the first 10 minutes of exposure to KCL.

Conclusion：During acute pneumonia a calcium overload and a decrease of the ability to mobilize calcium occur to right ventricular myocytes.

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