PROTECTIVE EFFECT OF BAICALIN ON THE OXIDATIVE TOXICITY OF NEURONAL AND ASTROGLIAL CELLS Song YZ, Yang
YJ, Jia YJ Department of Pediatrics, Xiangya Hospital, Changsha,
China Objective: To explore the effect of baicalin, a flavonoid extracted from the
radix of Scutellaria baicalensis Georgi,
on neuronal and astroglial cells viability, and their survival against
oxidative injury. Method: Neuronal and astroglial cells from the forebrains of SD rat pups were
cultured, treated with series concentrations of baicalin (2.5~40μM), and cells viability
was assayed by MTT, respectively; After preincubation with series
concentrations of baicalin (2.5~40μM) followed by treatment
with H2O2 (80μM to neuron, and 160μM to astrocytes), MTT
assay was performed to determine cell survival. Results: The viability difference among groups of neuronal cells, or of
astroglial cells, was not significant statistically, either (F=0.49, and
1.001, respectively, both P>0.05), when treated with series concentrations
of bacalin. After preincubated with bacalin, both neuronal and astrocytic
survial against oxidative stress was improved significantly (F=24.384, and
3.752, respectively, both P<0.01), and this protective effect was in a
dose-dependent manner. Conclusions: In the concentration range 2.5~40μM, baicalin was toxic to neither neuronal nor astroglial cells. However, this flavonoid can protect neuron and astrocytes against oxidative stress-induced injury dose-dependently. |
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