HL-60 CELL
DIFFERENTIATION INDUCED BY NITRIC OXIDE DONORS IN VITRO
Zhao YH, Luo CH, Liao
QK, Yuan XJ, Yang XJ
Department of
Pediatrics, Second University Hospital, West China Medical Center of
Sichuan University, Chengdu, China
Objective: To investigate HL-60 cell differentiation
induced by nitric oxide (NO) donors.
Methods: In vitro incubation of HL-60 cells with
NO donors, sodium nitroprusside (SNP) and S-nitrosoacetyl penicillamine
(SNAP), as well as potassium ferricyanide (PFC), SNP analogue, at the final
concentration of 0.1, 0.5, 1.0 mmol/L for 5 days. Cell differentiation was
observed by cytomorphology and nonspecific esterase (NSE). Cell-surface
antigen CD14, CD33 expression was analyzed by flow cytometry.
Results: 79.2% of HL-60 cells were induced into
more differentiated granulocytes by 1.0 mmol/L SNP, and their growth were
inhibited. For NSE staining, 56.8% of cells were positive, as compared with
55.5% in controls (P > 0.05), and rate of inhibition was no more than
50%. Flow cytometry analysis showed that expression of CD14 decreased. SNAP
and PFC had no effects on the cell differentiation.
Conclusion: NO can induce HL-60 cells to
differentiate along the granulocytic pathway. The differentiation is
related with NO sources and their concentration.