A MOLECULAR EPIDEMIOLOGY STUDY ON MOTHER-TO-CHILD TRANSMISSION OF TRANSFUSION TRANSMITTED VIRUS Zhou XG1, Xiao X2, Zhu
LH1, Xiong AH2, Chen X2, Xiao XM2,
Zou BP3, Xu LM3, Mao XJ2. 1Maternal and Child Health
Hospital of Guangdong Province, Guangzhou, China 2The First Affiliated
Hospital of Medical College, Jinan University, Guangzhou, China 3Shenzhen Research
Institute of Hepatology, Shenzhen, China Objective: To investigate
transmitted virus (TTV) infectious rate in pregnant women and explore the
route and transmission rate of mother-to-child transmission. Methods: A nested polymerase chain
reaction (n-PCR) technique was established to detect TTV DNA in 490 pair of
maternal sera of pregnant women and umbilical blood of their infants in
Guangzhou city. PCR products from the 8 women and their infants with TTV DNA
positive were cloned and sequenced to confirm its specificity. Results: TTV DNA was detected in 87
mothers and 12 their babies, respectively. TTV DNA positive rate in pregnant
women was 17.8%,and its mother-to-child transmission rate was 13.8%. The homology of
Guangzhou isolate and Japanese TTV was 85.3%~98.2% at both nucleotide
and amino acid levels. Conclusion: The results show that TTV infection is common in pregnant women population and TTV can be transmitted into their babies via placenta (vertical transmission). This Guangzhou TTV isolate and that reported by Japan all belong to a same genotype. |
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