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STUDY OF UMBILICAL CORD BLOOD TRANSPLANTATION IN IMMUNODEFICIENT MICE MODEL

Wang J X, Lan J C, Liu C L et al.

General Hospital of Lan Zhou Military Area Command Lan Zhou, China

 

Objective: To observe the effects of umbilical cord blood transplantation in immuno-deficient mice and consult for clinical transplantation as well as the funding of umbilical cord blood bank.

MethodsTo transplant mononuclear cell, or mononuclear cell freezed in liquid nitrogen, or mononuclear cell with plasma which all came from human umbilical cord blood, to immunodeficient mice, which had received a lethal dose 60Co-rays radiation the establishment and reconstruction of stem cell were observed.

Result: The survival transfusion of umbilical cord blood is 1.0~2.0*106 cell per mice both fresh and freezed cells. But there shows some difference in stem cell estabishment between fresh and freezed cord blood.

Conclusion: Human umbilical cord blood riched with stem cells can be successfully transplanted into mice and reconstruct blood in mice, the stem cells number in one umbilical cord blood can be transplanted into one child who weights not more than 30kg. Transfusion of umbilical cord blood plasma is benifit for recipient in blood recovery.