0263
EFFECT OF TRANSPLANTATION OF GENETICALLY MODIFIED MYOBLASTS
PRODUCING BDNF ON MEMORY OF NEONATAL RATS SUBJECTED TO HYPOXIC-ISCHEMIC
ENCEPHALOPATHY Hong XR1, Shi
J2, Qu S3, Lu XX1 and Chen XM1 1 Department of Pediatrics,
Fuzhou General Hospital, Fuzhou, China 2 Department of
Neurobiology, Shanghai Second Medical University, Shanghai, China 3 Department of
Neurobiology, Second Military Medical University, Shanghai, China Objective: To explore the effect of cerebral
transplantation of genetically modified myoblasts producing brain-derived
neurotrophic factor (BDNF) on memory in neonatal rats subjected to
hypoxic-ischemic encephalopathy (HIE).
Methods: Fifty-eight pups from 6
litters weighing 13.3～17.8 g were randomized into sham-operated
group (C, 11 pups), HIE+BDNF transplantation group (B, 21 pups) and
HIE+mock-transplantation group (A, 26 pups). A genetically engineered rat myoblast cell line that
is confirmed to express biologically active BDNF was developed. A
unilateral stereotaxical intracerebroparenchymal transplantation of either BDNF（+）/L-6TG (group B) or BDNF（-）/L-6TG (absence of BDNF,
group A) at 0.8μl of cell suspension (4×104/μl) into the left cortex
of the brain was carried out shortly after HIE undergone by ligation of
left common carotid artery followed
by a 2.5 h inhalation of humidified 8% O2+92% N2 at
37℃. The location of microinjection in relation to lambda was 2.1 mm
rostral, 1.5 mm lateral to the left, and 1.5 mm deep to the skull surface.
Changes of memory were investigated 6 weeks after the procedure by the
tests of active avoidance response, one-trial passive avoidance response. Results: There were significant
increases of abilities of acquirement and maintenance of active avoidance response and one-trial passive avoidance response
in group B when compared with group A, although most parameters of either
group B or group A were lower than those of group C. Conclusion:
Present
data suggest that cerebral transplantation of genetically modified
myoblasts producing BDNF promotes the recovery and enhances the development
of brain memory function after HIE.