0264
EFFECTS OF INTRACEREBRAL TRANSPLANTATION OF
GENETICALLY MODIFIED MYOBLASTS PRODUCING BDNF ON DIFFERENT BRAIN REGIONS OF
NEONATAL RATS SUBJECTED TO HYPOXIC-ISCHEMIC ENCEPHALOPATHY Hong XR1 and Shi J2 1 Department of
Pediatrics, Fuzhou General Hospital, Fuzhou, China 2 Department of
Neurobiology, Shanghai Second Medical University, Shanghai, China Objective: To investigate the neuroprotection
of
different brain regions after transplantation of genetically modified
myoblasts producing brain-derived neurotrophic factor (BDNF) into cortices
of neonatal rats subjected to hypoxic-ischemic encephalopathy (HIE). Methods: Seven-day-old pups were randomized into
sham-operated group (C), HIE+BDNF group (B) and HIE+mock-transplantation
group (A). A rat myoblast cell line expressing and secreting BDNF (BDNF（+）/L-6TG) was constructed.
A stereotaxical intracerebroparenchymal transplantation of either BDNF（+）/L-6TG (B) or BDNF（-）/L-6TG (absence of BDNF,
A) at 0.8μl of cell suspension (4×104/μl) into the left cortex
of the brain was carried out shortly after HIE undergone by ligation of left common carotid artery followed
by a 2.5 h inhalation of humidified 8% O2+92% N2 at
37℃. Changes of areas of different brain regions of cortex,
hippocampus and striatum at different sections were observed 21 d after the
procedure. Injury severity scores of brain tissues were also performed. Results: Twenty-one days after the manipulation, various
degrees of atrophy were observed in the three regions of left hemispheres
in group A or B versus contralateral corresponding regions or group C
whereas this atrophy in group B was significantly reduced when compared to
group A. Not only was the atrophy of cortex near the grafting point
alleviated but some other regions of left hemispheres. A similar change
pattern was seen in injury severity scores of the tissue. Conclusion: Present data suggest that intracerebral
transplantation
of genetically modified myoblasts producing BDNF has a beneficial effect on the protection from damage of both circumscriptus brain
tissues and some other areas distanced from the
grafted place.