0268

APPLICATION OF SDS CHEMICAL METHOD TO EXTRACT OUTER MEMBRANE PROTEINS OF ACINETOBACTER CALCOACETICUS FOR EPIDEMIOLOGIC INVESTIGATION

Zhang Wanming, Wu Shixiao, Liu Guanxin

Hubei Medical Staff College, Jingzhou,.China

Objective: To compare with the results in extracting bacteria outer membrane proteins (OMP) between SDS chemical method and ultrasounic physical method in investigating the epidemiology of A calcoaceticus sepsis in the new-born.

Methods: We used SDS chemical method to break the cell wall of Acinetobacter calcoaceticus for extracting their outer membrane proteins and then performed SDS-PAGE to investigate the epidemiology of A.calcoaceticus sepsis in newborn infants, so did ultrasounic physical method.

Results: SDS chemical method was quite satisfactory. Ultrasounic physical method to break the cell wall of bacteria for OMP extraction needs refigerated ultracentrifuge (147 000xg) which very expensive, while SDS chemical method only needs high speed centrifuge (38 000xg) which is much cheaper .

Conclusion: SDS chemical method is easy to popularized.