EVALUATION OF EBV-IgM DETECTED BY ELISA FOR
DIAGNOSIS OF CHILDREN INFECTED WITH EBV
Chen Huanhui, Chang Ruxu, He
Cuijuan
Guangzhou Children’s Hospital,
Guangzhou, China.
Objective: The
aim of this study is to evaluate diagnostic significant of serum the EBV-IgM detected by ELISA in
the children infected with EBV.
Material and Methods: In
this study, we examined sera from the 790 hospitalized children aged
between 4 days and 14 years for viral capsid antigen specific
antibodies—EBV IgM. We also tested 38 paired sera for EBV-IgG EBNA and 17
paired sera were test for virus by PCR. EBV-IgM were measured by ELISA;
EBV-IgG were detected by immunofluorescence.
Result: in the
total 790 patients, 124 (15.70%) cases were detected to be EBV-IgM
positive. Among them we found EBV-IgM positive in 19 (86.36%) of 22 patients with infectious
mononucleosis(IM), 8 (16.33%) of 49 patients with Idiopathic
thrombocytopenic Purpura (ITP), 6 (7.89%) of 76 patients with
Henoch-Schonlein Purpura (HSP), 19 (15.32%) of 124 patients with viral
encephalitis, 23 (15.54%) of 148 patients with bronchitis, 3 (14.29%) of 21
patients with tonsillitis, 25 (13.66%) of 183 patients with
bronchoalveolitis，10 （12.20%）of 82 patients with upper respiratory tract
infection, 4 (19.04%) of 21 patients with anemia, 1 (4.76%) of 21 patients
with other encephalopathy. Compared the ELISA EBV-IgM with the traditional
diagnostic method in which the convalescent serum IgG titer has fourfold
higher than acute phase serum or acute phase serum is negative but
convalescent serum is positive; PCR positive, 25 of 38 paired were detected
to be negative and 7 of 38 paired sera to be positive by two method. 84.2%
(32/38 case) of ELISA EBV-IgM coincide with those of traditional method,
the sensitivity of EBV-IgM is 77.8% and specificity is 86% .
Conclusion: ELISA EBV-IgM is a sensitive and specific index
for EBV infection and can be use for early diagnosis of children infected
with EBV.