RELATIONSHIP BETWEEN BIOLOGICAL EFFECTS AND UPTAKE OF C-MYC ANTISENSE
OLIGONUCLEOTIEDS IN ERYTHROLEUKEMIC CELLS YUAN XJ, ZHOU M, LUO CH, LIAO QK Pediatric Department, 2nd
University Hospital, Sichuan University (Huaxi Campus), Chengdu, China Objective: To study the relationship
between biological effects and cellular uptake of c-myc antisense
oligonulceotides and their cationic liposome complexes in human
erythroleukemic K562 cells. Methods: The biological activity of
c-myc antisense olignoucleotides and their liposome complexes was determined
by counting the cell number using MTT assay, while their cellular uptake
conditions were measured by lazer confocal scanning technique. Results: Proliferation of K562 cells was inhibited by antisense
S-oligo and PS-oligo, while antisense P-oligo and sense S-oligo were
ineffective. In oligo-liposome complexes group, inhibiting degree of K562
proliferation was greatly increased comparing with oligo group. In confocal
fluorescence microscopic study, uptake of S-oligo, PS-oligo and P-oligo was
observed to be time-dependent and mainly distributed on the cell surface and
in the cytoplasm, when complexed with liposomes, cellular uptake was
significantly increased and the fluorescence was located mainly in the
nucleus. Conclusions: Control of the intracellular uptake and localization of antisense oligo nucleotides in K562 cells by cationic liposomes may lead to a more efficient antisense activity. |
0300