0313

CHANGES AND SIGNIFICANCE OF CIRCULATING ENDOTHELIAL CELLS AND ACTIVATED PLATELETS IN MECONIUM ASPIRATION SYNDROME INFANTS

Zhang F-X, Wang S-Y, and Liu B

Chao Yang Hospital, Beijing, China

Objective: The article discusses the changes and clinical significance of the rate of circulating endothelial cells and platelets activation in Meconium aspiration syndrome (MAS).

Method: The circulating endothelial cells and activated platelets are marked by fluorescent monoantibodies rats-antihuman CD45-PE, CD31-FITC, CD62P-PE, IgGI-FITC and IgGI-PE made by the PHARMINGEN Company and are tested with FACS Calibur flow cytometry made by the U.S. Becton Dickinson Co.

Results: Both the rate of circulating endothelial cells and of platelets activation are higher in MAS infants than in the comparision group (P<0.001), and the rates increase significantly when the oxygen-deficiency time lasts.

Conclusion: MAS can damage the blood vessel endothelium of neonates and cause an increase in platelets activation rate. Intensified tending and immediately treatment of intertemple embarrassed fetus is essential. The respiratory tract of the infants should be cleaned up duly after delivery. Filthy stuff like amniotic fluid and meconium should be drawn out with windpipe tubes. Lung irrigation should be performed when necessary to avoid oxygen-deficiency and to decrease the damage of blood vessel endothelium.

[Key words]: Meconium aspiration pheumonia; Circulating endothelial cell; Platelets activation rate