0338

ANTIMULLERIAN HORMONE IN BOYS WITH GROWTH HORMONE DEFICIENCY ANDHYPOGONADOTROPIC HYPOGONADISM

Wang Wei, Wang Defen, Ni Jihong,Sun Wenxin, Wang Xiumin, Fu Hongmei

Department of Pediatrics, Ruijin Hospital, Shanghai Second Medical University, Shanghai, China

AIMS: To evaluate the serum testosterone regulatory mechanism of AMHsecretion at puberty and to determine the AMH measures in clinicalapplications

METHODS: 48 health adolescent boys in various genitalia Tanner stages fromG1~G4-5 (control group) and 10 male patients aged 12~20 years old with completeGHD and hypogonadotropic hypogonadism (patient group) were assayed for serumAMH, T, LH and FSH. All patients were enrolled in Genotropin (0.1U/kg/d) treatment for 2 years with combined HCG (1000u Biw) or Testorison Depot (100mg/m) treatment in the 2ndyear. Blood samples were collected atstart and thereafter every 6 months.

RESULTS: ①In control group, Serum AMH levels were highest in prepuberty,declined subsequently according to advancement of pubertal stage, being lowestin stage G4~5 (F=16.78, P<0.0001), and in negative correlation withconcentration of T (r=-0.49, P<0.0001);②The patient group evaluated serum AMH levels as 318.0±34.7pmol/L at pretreatment, significantly higher than the normalcontrols (32.75±14.6pmol/L) (t=-8.15, P<0.0001). After the first year of rhGHtherapy, mean serum level of AMH declined to 195.4±61.2pmol/L (t=4.01, P<0.005). A further decrease of serum AMHlevels were observed at the end of the 2ndyear (by combinedtreatment for 1 year), also significantly different to levels before using sexhormones (t=3.511, P>0.01). Serum T levels elevated in contrast to thedecreased AMH concentration.

CONCLUSION: ①Serum AMH levels evaluate negative correlation with serum Tconcentration in normal adolescent boys.②Serum AMH reveal high values in GHD patients with hypogonadotropichypogonadism, but decline by the rhGH and sexual hormone treatment withconcomitant elevation of T concentration.③Measurement of serum AMH may provide additional functional test fortestis by estimation of Sertoli cells as well as Leydig cellsfunctions.