MECHANISM UNDERLYING THE AMELIORATIVE EFFECTS OF NGF
ON NEWBORN RAT WITH HYPOXIA-ISCHEMIA BRAIN DAMAGE
Wang LS,
Zhu CL, Cheng XY
Department
of Pediatrics The Third Affiliated Hospital, Medical College of Zhengzhou
University, Zhengzhou, China
Objective: To explore the mechanism
of ameliorative effects of nerve growth factor (NGF) on newborn rat with
hypoxia-ischemia brain damage (HIBD).
Methods: NGF dosage of
0.0001mg/kg and 0.0003mg/kg were administrated intraperitoneally to the rat
pups 30 minites before the operation and immediately following the hypoxic
exposure. Percentage of brain water content was determined by dry/wet
weight ratio and brain tissue calcium concentration was measured with
atomic absorption spectroscopy. The monoamine neurotransmitters
concentration in the rat brain were measured using high performance liquid
chromatography with electrochemical detector (HPLC-ECD).
Results: The contents of brain
water, calcium concentration and monoamine neurotransmitters in the brain
tissues increased significantly after hypoxic ischemic insult in the
ipsilateral hemisphere of HIBD group, compared with the control group and
NGF groups. There were significant discrease of water content, calcium
concentration and monoamine neurotransmitters in HIBD group after NGF was
used.
Conclusions: Water content, calcium
concentration, and monoamine neurotransmitters may alone or interactive
each other involved in the pathophysiologic process of HIBD. NGF had
ameliorative effects on the newborn rat with HIBD, the possible mechanism
involved in maintaining calcium homeostasis, ameliorating brain edema and
attenuating or inhibiting the neurotoxic effects of monoamine
neurotransmitters.