MECHANISM UNDERLYING THE AMELIORATIVE EFFECTS OF NGF ON NEWBORN RAT WITH HYPOXIA-ISCHEMIA BRAIN DAMAGE

Wang LS, Zhu CL, Cheng XY

Department of Pediatrics The Third Affiliated Hospital, Medical College of Zhengzhou University, Zhengzhou, China

 

Objective: To explore the mechanism of ameliorative effects of nerve growth factor (NGF) on newborn rat with hypoxia-ischemia brain damage (HIBD).

Methods: NGF dosage of 0.0001mg/kg and 0.0003mg/kg were administrated intraperitoneally to the rat pups 30 minites before the operation and immediately following the hypoxic exposure. Percentage of brain water content was determined by dry/wet weight ratio and brain tissue calcium concentration was measured with atomic absorption spectroscopy. The monoamine neurotransmitters concentration in the rat brain were measured using high performance liquid chromatography with electrochemical detector (HPLC-ECD).

Results: The contents of brain water, calcium concentration and monoamine neurotransmitters in the brain tissues increased significantly after hypoxic ischemic insult in the ipsilateral hemisphere of HIBD group, compared with the control group and NGF groups. There were significant discrease of water content, calcium concentration and monoamine neurotransmitters in HIBD group after NGF was used.

Conclusions: Water content, calcium concentration, and monoamine neurotransmitters may alone or interactive each other involved in the pathophysiologic process of HIBD. NGF had ameliorative effects on the newborn rat with HIBD, the possible mechanism involved in maintaining calcium homeostasis, ameliorating brain edema and attenuating or inhibiting the neurotoxic effects of monoamine neurotransmitters.

 

 

 
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