0354

CARBONYL IRON MAGNETIC FRACTIONATION CONCENTRATES HEMATOPOIETIC PROGENITOR/STEM CELLS FROM G-CSF MOBILIZED PERIPHERAL BLOOD

Tang Y-M¹, Billups C² and Reynolds C-P²

¹ Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China

² Children's Hospital Los Angeles, Los Angeles, USA

Objective: To explore the possibility of using a simple way to remove engraftment-unrelated cells before immunomagnetic purging or CD34 positive selection to reduce the cost of those procedures.

Methods: Using sterile carbonyl iron (CI) particles and high-energy samarium cobalt magnets to remove the phagocytic cells from G-CSF (granulocyte-colony stimulating factor) mobilized peripheral blood progenitor/stem cells (PBSC). A model system was used to evaluate the efficacy of neuroblastoma purging. A flow cytometry and a colony assay were used for CD34+ cell enumeration and colony-forming unit-granulocyte / macrophage (CFU-GM) evaluation, respectively.

Results: After a short period incubation of PBSC with CI followed by the magnetic removal, cell numbers can be reduced to 34.0%±11.8% (n = 10, P < 0.00001). CD34⁺ cell recoveries were 84.3%±50.3% (n = 10, P = 0.09) with a 2.6±1.5-fold increase (P < 0.00001). CFU-GM colony recoveries were 100.0%±37.4% (n = 7, P = 0.998) with a 3.0±1.1-fold enrichment (P < 0.00001). The efficacy of purging neuroblastoma cells seeded into PBSC (n = 3, P = 0.44) was not compromised by CI fractionation.

Conclusions: Carbonyl iron fractionation is a rapid and an effective means of concentrating hematopoietic progenitors / stem cells from PBSC without compromising their activities. This method warrants further testing on clinical trials of stem cell enrichment before immunomagnetic purging or CD34 positive selection using CliniMACS.

Abstract Title: CARBONYL IRON MAGNETIC FRACTIONATION CONCENTRATES HEMATOPOIETIC PROGENITOR/STEM CELLS FROM G-CSF MOBILIZED PERIPHERAL BLOOD

Authors: Tang Y-M, Billups C and Reynolds C-P

Specialty: Hematology / Oncology

Address: #57 Zhuganxiang, Yan-an Road

City: Hangzhou Country: P R CHINA Zip Code: 310003

Telephone: (work) +86 (571) 7061007 ext 2460 Fax: +86 (571) 7033296

(home) +86 (571) 5390411

E-mail: BBQTY@mail.hz.zj.cn

Presenting Author

Name: Tang Y-M Signature: Tang Yongmin

This is a submitted abstract Ö . (Please tick.)

Preferred presentation format: Ö podium (oral);

Preferred Session: 19