INHIBITION OF THE GENE EXPRESSION OF VIRUS IN STEROID RESPONSIVE SIMPLE NEPHROTIC SYNDROME BY ANTISENSE OLIGONUCLEOTIDES

Zhu X-S, Wang Z,Li C-S,Tao Y-H

Department of pediatrics,the Second Hospital, West China Medical Center, Sichuan University, Chengdu, P.R.China

 

Objective: By inhibition of the gene expression of respiratory tract viruses in steroid responsive simple nephritic syndrome (SRSNS) and by antisense oligonucleotides (ASONs), we have attempted to provide more proof for the infection of respiratory tract viruses in SRSNS and the viruses triggering the disease and to offer a new path to the treatment clinically.

Methods: After transfection by ASONs to peripheral blood mononuclear cells (PBMCs),the gene expression of respiratory tract viruses was detected by RT-PCR; the expression of NF-κB was detected by electronphoretic mobility shift assay (EMSA).

Results: 1.After transfection by ASONs to PBMCs, the gene expression of respiratory tract viruses was inhibited significantly in SRSNS in active stage compared with control and transfection by sense oligonucleotides (SONs).(P<0.05)

The positive rate of the gene expression of respiratory tract virus was increased significantly in SRSNS in active stage (including respiratory syncycial viruses 31.3%, influenza viruses 18.8%) compared with in remission, the other types of glomerular disease and normal control.(P<0.05)

2.After transfection by ASONs, the expression of NF-κB was inhibited significantly in SRSNS in active stage compared with control and transfection by SONs.(P<0.05)

Conclusion: The study demonstrated that the infection of respiratory tract viruses actually exist in SRSNS in active stage, and the gene expression of the viruses was inhibited by ASONs that may result in the activation of NF-κB way was interrupted .Therefore we suggested that the infection of respiratory tract viruses be one of the triggers in onset of SRSNS.

Keyword:  antisense; oligonucleotides; respiratory; tract; viruses; steroid; responsive; simple; nephritic; syndrome

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