ROLE OF NF-κB
IN RAT BONE MARROW STROMAL CELLS DIFFERENTIATING INTO NEURONS INDUCED BY
BAICALIN
Jia YJ, Yang YJ, Song YZ
Xiangya Hospital, Central South University, Changsha, China
Objective: To explore whether the transcription factor
NF-κB takes
part in bone marrow stromal cells (MSCs) differentiating into neuron
induced by baicalin.
Method: MSCs from adult rats were induced by baicalin
in serum-free medium for 6h to 8h. The untreated cells, as control group,
were only induced in serum-free medium without baicalin. The activity of
NF- κB
was measured by the presence of the NF-κB subunits RelA (p65) translocated into the nucleus by indirect
immunofluorescence. The positive percentages of neuron-specific enolase (NSE),
200-kilodalton neurofilament (NF) protein expression, both of neuron specific protein, were
measured by immunocytochemistry ABC staining.
Results: Indirect immunofluorescence showed
that the activity of NF- κB correlated with 71.8%±5.3% p65 in nuclei region of untreated cells. And in treated
cells by baicalin, 85.2%±6.0% p65 retained in the cytoplasm. Moreover, immunocytochemistry
showed that the positive percentages of
NSE and NF protein expression in treated cells were 34.3%±14.2 % and 28.5%±9.4%, respectively. And no NSE or NF
positive cells were found in untreated cells.
Conclusions: The data presented support that baicalin
treatment inhibits the cleavage and translocation of NF-κB from the
cytoplasm to the nucleus, which may be involved in MSCs differentiating
into neuron.