QUANTITY-COURSE AND
MECHANISM OF BAICALIN PROTECTION BRAIN SLICE OF RAT FROM PERTUSSIS BACILLI
YIN F, YANG YJ, YU PL, et al.
Department of Pediatrics, Xiangya Hospital, Changsha,
China
Objective:
To observe the Quantity-course and mechanism of baicalin protection brain
slice of rat from Pertussis Bacilli.
Method: Brain
slices were randomly divided into 7 groups: Normal slice culture group (NC
group); 10% Pertussis Bacilli (PB) slice culture group (PBC group); 10% PB slice culture group
after pretreatment with baicalin
(BPBC group); glutamate slice culture group (GLC group); glutamate
slice culture group after pretreatment with baicalin (BGLC group); H2O2
slice culture group (HOC group); H2O2 slice culture
group after pretreatment with
baicalin (BHOC group). BPBC group could be divided into 7 subgroups
according to quantity of baicalin .
Results:
The concentrations of LDH in PBC group (15.10±4.89 u/g protein), GLC group (15.49±5.66)and HOC group (16.54±5.47) were markedly increased than that of NC group (6.10±2.87) (P< 0.01). The content of LDH was significantly
decreased in BGLC group than in PBC group (P< 0.01), And decreased in BGLC group, BHOC group
than in GLC group as well as HOC group (P< 0.01).
Conclusions: The result suggested that baicalin has a
protective effect on the pertussis bacilli-induced damage in rat brain
slice, and protective effect was related to decreasing damage by glutamate
and H2O2.