QUANTITY-COURSE AND MECHANISM OF BAICALIN PROTECTION BRAIN SLICE OF RAT FROM PERTUSSIS BACILLI

YIN F, YANG YJ, YU PL, et al.

Department of Pediatrics, Xiangya Hospital, Changsha, China

 

Objective: To observe the Quantity-course and mechanism of baicalin protection brain slice of rat from Pertussis Bacilli.

Method: Brain slices were randomly divided into 7 groups: Normal slice culture group (NC group); 10% Pertussis Bacilli (PB) slice culture group  (PBC group); 10% PB slice culture group after pretreatment with baicalin  (BPBC group); glutamate slice culture group (GLC group); glutamate slice culture group after pretreatment with baicalin (BGLC group); H2O2 slice culture group (HOC group); H2O2 slice culture group after pretreatment with baicalin (BHOC group). BPBC group could be divided into 7 subgroups according to quantity of baicalin .

Results: The concentrations of LDH in PBC group (15.10±4.89 u/g protein), GLC group 15.49±5.66and HOC group 16.54±5.47 were markedly increased than that of  NC group 6.10±2.87 (P< 0.01). The content of LDH was significantly decreased in BGLC group than in PBC group (P< 0.01), And decreased in BGLC group, BHOC group than in GLC group as well as HOC group (P< 0.01).

Conclusions: The result suggested that baicalin has a protective effect on the pertussis bacilli-induced damage in rat brain slice, and protective effect was related to decreasing damage by glutamate and H2O2.

 

 
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