ASSOCIATION BETWEEN GENETIC POLYMORPHISMS OF THE
INTERLEUKIN-10 GENE PROMOTER AND CLINICAL PHENOTYPES IN CHILDHOOD ASTHMA IN
CHINA
Zhang JL1, Chen H2, Hu LP3,
Fu J1, Zhang HQ1, Chen YZ1
1 Capital Institute of
Pediatrics, Beijing, China
2 Chinese Academy of
Medical Sciences, Beijing, China
3 Chinese Military Academy of Medical
Sciences, Beijing, China
Objective: To determine whether genetic polymorphisms in the promoter
region of the interleukin-10 (IL-10) gene affected clinical phenotypes and
asthma susceptibility in childhood asthma in china.
Methods: One hundred and
sixty-eight unrelated childhood asthma cases and 70 normal control children
were studied. The total serum IgE、prick skin test (PST) to
20 common allergens and FEV1 were measured as clinical criteria. Three
polymorphisms (G or A at position –1082, C or T at position –819 and C or A
at position-592) in the promoter region of the IL-10 gene were genotyped by
polymerase chain reaction and restriction endonuclease digestion.
Results: The level of total
serum IgE was showed significant difference among three genotypes of –1082
and –819 polymorphic sites by MIXED procedure analysis (p=0.005 and
p=0.0161 respectively). G allele at –1082 locus and T allele at –819 locus
were associated with elevated total serum IgE (p<0.01, OR=3.16;
p<0.05, OR=1.84). None of three IL-10 promoter polymorphisms was
associated with PST and FEV1 phenotypes. There was no significant change in
the allele frequencies of the three loci on the IL-10 gene promoter region
in asthmatic patients with control group. However, we found that the most
common haplotypes such as GTA, GCC, GTA/GCA and ACC/GCC were markedly
different between childhood asthma and controls (c2=8.546,
p<0.05).
Conclusion Polymorphisms within the
IL-10 gene promoter that are associated with elevated total serum IgE may
contribute to the development of the asthmatic phenotype.