DYNAMIC
CHANGES IN CARDIAC TISSUES NO MDA AND SOD IN NEONATAL RATS WITH ENDOTOXIC
SHOCK
Du XH, Jia XZ, Wu YB and Han YK
Pediatric Department of the 2nd Clinical
College,
China Medical University, Shenyang, China
Objective: To observe
the dynamic changes in cardiac tissues nitric oxide (NO), malondiadehyde
(MDA) and superoxide dismutase (SOD) after endotoxic shock, and to explore
their potential role in the pathogenesis and dexamethaxone (Dex) effect on
endotoxic shock in neonatal rats.
Methods: health
seven-day-old newborn wister rat (n=175,each group7) were involved in this
study. The agents given by intraparitoneal injection include: Group1:
Control group, Group2: (LPSgroup) LPS5mg/kg, Group3,4and5 (Dexgroup)
LPS5mg/kg+Dex, 5,10,15mg/kg, respectively. NO, MDA and SOD of cardiac
tissues (CT) were measured at the time points of 0,2,4,6,24 hour after
injection, respectively.
Results: (1) In LPS
group, Cardiac tissues NO and MDA level markedly increased at 4h(P<0.01)
and peaked at 24h(P<0.01), respectively. The activity of SOD
significantly decreased (P<0.01) at 4h until to 24h. (2) In Dex group,
the concentration of NO and MDA in group 3 and group 4 also elevated, but
markedly less than that in LPS group. The activity of SOD decreased partly.
In group 5, the levels of three items above was as the same as the control
group.
Conclusions: The study
suggest NO, MDA and SOD are involved in pathophysiological processes and
Dex inhibiting NO and MDA secretion and enhancing SOD activity following
endotoxin challenge in a dose-dependent manner.