THE OBSERVATION OF PPD STIMULATED THE LEVEL OF
IL-12, IL-4, IFN-Γ IN PBMC FROM CHILDREN WITH ASTHMA
LI M-R , LI C-C, CHEN X-F, LI Y-C
The Yu Ying Children’s Hospital of Wenzhou Medical College,
Wenzhou, China
Objective: To explore the relationship PPD stimulated with IL-12, IL-4, IFN-γ
level in children with asthma.
Methods: PBMCs from 24 cases of children with asthma and the 18 cases of
health controls were isolated and were cultured in flat-bottomed 24 well
culture plates at a concentration of 2×106 per well in the presence of PPD(50μg/ml) for 24h at 370c with 5%CO2
.The level of IL-12, IL-4, IFN-γ in the culture supernatant was estimated
by ELISA.
Results: The amounts of IL-12 and IFN-γin PBMC with PPD stimulation from asthmatic patients were greater
than those in PBMC with
the health controls
(P<0.01).
Conclusion: The IL-12 and IFN-γshowed lower level in asthmatic
patients , suggesting the Th2 responses is predominant and the problem of antigen-presenting
course existed .