Detection of cytomegalovirus antigen in urine and nephric tissue by immunohistochemistry and its clinical significance in children with hematuria

Luan J-W, Wu Y-X, Xiong S-Y, et al.

Children¡¯s Hospital of Wuhan, Wuhan, China

 

Objective: To investigate the infection of cytomegalovirus in children with hematuria.

Method: Urine of 55 infants with hematuria were used to detect cytomegaiovirus early antigen (CMVEA) by immunohistochemistry  (streptavidin-peroxidase or S-P method). Six of these infants received renal  biopsy and were detected CMVEA in their nephric tissue.

Result: The positive rate of CMVEA in children with asymptomatic hematuria was 80% (32/40),while the positive of CMVEA in nephritis group and control group were 20% (3/15) and 18.9% (10/53).The study showed that the positive rate of CMVEA in group of asymptomatic hematuria was markedly higher than in group of nephritis and control(p<0.005,p<0.05)Five of six children who received the renal biopsy were detected the CMVEA in their nephric tissue,the positive rate was 83%(5/6).Most of CMVEA in renal tissue were founded in the cells of cortical collecting tubule.

Conclusion: The primary pathogeny of asymptomatic hematuria likely is the infection of cotymegalovirus.The nephrosis induced by infection of CMV is commonly not serious: the swell and vacuolization of tubule were seen, while the pathological changes of glomerul and interstitium are inconspicuous.

 

 
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