EXPRESSION OF CELLULAR INHIBITOR OF APOPTOSIS PROTEIN 2 MRNA IN
HUMAN SUBCUTANEOUS AND OMENTAL ADIPOSE TISSUE
Li
X-N, Chen R-H, Liu
Q-Q, Chen Y-J,
et al.
Nanjing Medical University, Nanjing, China
Objective: The aim of this study is to investigate the possibility that
the tissue distribution and
quantification of the mRNA of
cIAP-2 expressed in
human adipose tissues and
related to age and BMI ,thus demonstrating a further mechanism by
which they regulated human adipocytes.
Methods: Biopsies from omental and abdominal subcutaneous adipose tissue
are obtained from 18 patients (11 adults, 7 children. BMI 5~26kg/m2)
under going elective open-abdominal surgery. None of patients had diabetes
or severe illness, and none were on medications known to influence adipose
mass or metablism. Adipose tissue RNA is extracted by guanidinium
thiocyanate-phenol technique. Gene expression by RT-PCR .Compare level of
candidate mRNA expression in paired Sc and Om adipocytes, obesity and lean,
different age, calculate the correlation coefficient between BMI and level
of mRNA expression .
Results:
cIAP-2 was expressed at higher levels in abdominal subcutaneous than omental adipose (Sc＞Om in 17 subjects, mean Sc:Om
ratio 2.83±0.45, P＜0.01 ＝ , cIAP-2 is not corelation
with BMI and age (r = 0.23,P＞0.05, r=0.31 p＞0.05).
Conclusion: The cIAP-2 is one of a number of
mammalian proteins, known as inhibitors of apoptosis (IAPs), that suppress
apoptosis. cIAP-2 may be involved in the regulation of TNF-a signaling
.Because of the mRNA examined to date, cIAP-2 and TNF-a show consistent
site-related exprssion on human adipose tissue, andThus this depot-specific
differences may possible exist in the regulation of adipocyte apoptosis and
suggest that these molecules may have important roles in determining
functional properties particular to individual adipose depots.