EFFECTS OF INTRACEREBROVENTRICULAR INJECTION OF RECOMBINANT
LEPTIN ON FOOD INTAKE, BODY WEIGHT AND BLOOD LIPIDS IN DIET-INDUCED OBESE
RATS
Liu Q-Q, Chen R-H, Guo X-R, Fei L, Gong H-X
Nanjing Medical University, Nanjing, China
Objective:
To investigate the effects of intracerebroventricular injection of
recombinant murine leptin on body weight ,food intake and blood lipids in diet-induced obese rats and
normal rats.
Methods:
Male SD rats were fed a standard diet (n=15) and high-nutrition diet (n=20)
for 7 weeks .After 7 weeks , rats received cannulas implanted into the
right lateral ventricles. Allowed 7 days to recover , rats received an
intracerebroventricular injection of recombinant murine leptin (5 m g/rat)for 5 days. On each test day, the body weight and food intake
were measured before injection.
Blood lipids were measured after the experiment.
Results:
(1) The body weight in rats after fed high-nutrition diet was more
significantly increasing than that in normal rats (P<0.01). In
diet-induced obese rats, the inhibition of body weight and food intake were
observed after intracerebroventricular leptin injection at the first day (P<0.001).
But in normal group, the effects were observed after three days (P<0.05). (2) In obesity group with
leptin adiministration, the levels of TC, TG, LDL-C decreased significantly
(P<0.005). But there were no obvious changes in HDL-C in all groups.
Conclusions: Recombinant leptin could reduce the body weight, blood
lipids and inhibit the food intake in diet-induced obese rats after
intracerebroventricular injection.