EFFECTS OF INTRACEREBROVENTRICULAR INJECTION OF RECOMBINANT LEPTIN ON FOOD INTAKE, BODY WEIGHT AND BLOOD LIPIDS IN DIET-INDUCED OBESE RATS

Liu Q-Q, Chen R-H, Guo X-R, Fei L, Gong H-X

Nanjing Medical University, Nanjing, China

 

Objective: To investigate the effects of intracerebroventricular injection of recombinant murine leptin on body weight food intake and blood lipids in diet-induced obese rats and normal rats. 

Methods: Male SD rats were fed a standard diet (n=15) and high-nutrition diet (n=20) for 7 weeks .After 7 weeks , rats received cannulas implanted into the right lateral ventricles. Allowed 7 days to recover , rats received an intracerebroventricular injection of recombinant murine leptin 5 m g/ratfor 5 days. On each test day, the body weight and food intake were measured before injection.  Blood lipids were measured after the experiment. 

Results: (1) The body weight in rats after fed high-nutrition diet was more significantly increasing than that in normal rats (P<0.01). In diet-induced obese rats, the inhibition of body weight and food intake were observed after intracerebroventricular leptin injection at the first day (P<0.001). But in normal group, the effects were observed after three days P<0.05. (2) In obesity group with leptin adiministration, the levels of TC, TG, LDL-C decreased significantly (P<0.005). But there were no obvious changes in HDL-C in all groups.

Conclusions: Recombinant leptin could reduce the body weight, blood lipids and inhibit the food intake in diet-induced obese rats after intracerebroventricular injection.

 
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