STUDIES ON MECHANISM FOR BODY
WEIGHT MITIGATING BY NEUROPEPTIDE Y-Y5 RECEPTOR ANTISENSE
OLIGODEOXYNUCLEOTIDES
Gong H-X, Guo X-R, Chen R-H, Fei L, Mo B-Q
Nanjing Medical University, Nanjing, China
Objective: (1) To observe the influence of neuropeptide Y-Y5 Receptor Antisense Oligodeoxynucleotides (ODNs)
centrally injecting on food intake and the body weight of obese rats. (2) To study the effects of
NPY-Y5 antisense ODNs on white adipose tissue.
Methods: NPY-Y5
antisense ODNs
were centrally administrated in obese rats. Food
intake、body and
adipose tissue weight were observed. Average adipocyte cubic content was
calculated by MPIAS -500. And adipocytes apoptosis were detected by DNA gel
electrophoresis and TUNAL. The expressions of Bcl-2 and Bax genes
in adipose tissue were also detected by RT-PCR.
Results: (1) The food intake and the
body and adipose tissue weight were significantly inhibited. (2) The number
and cubic content of adipocytes were obveriously decreased. (3) The retroperitoneal
adipocyte cubic content decreased more significantly than in epididymal. And
the epididymal adipocyte was more sensible to apoptosis than
retroperitoneal adipocyte. (4) Bcl-2 gene expression decreased while Bax gene expression increased in adipotise tissue. (5) The serum
lipid profile showed a significant decrease in the concentration of
triglyceride (TG) and total cholesterol (TC), but has no marked influence
on high-density lipoprotein cholesterol (HDL-C). And the concentration of
insulin and leptin were also decreased.
Conclusion: NPY-Y5 receptor may be a key
mediator of NPY-induced feeding behavior. NPY Y5 receptor antagonist is a
promising new method for obesity therapy.