EXPRESSION OF Fas/FasL ON
PERIPHERAL BLOOD LYMPHOCYTES IN ASTHMA CHILDREN AND ITS SIGNIFICANCE
Yuan Y.S1, Gao L.N2, Wang L1
1. Department of
Pediatrics, The Affiliated Hospital of Hubei Medical Staff College,
Jingzhou, Hubei, China
2. Laboratory of
Immunology, The Second Hospital of Jingzhou, Jingzhou, Hubei, China
Objective: Because that
Fas/Fas ligand (FasL) can lead to T-cell apoptosis, we investigated
expression of Fas/FasL on peripheral blood T cells in asthma children and
sought to explore the relationship between its clinical significance.
Methods: Lymphocytes were
isolated with ficoll-hypaque gradient method and T lymphocytes were further
purified with nylon pili. Expression of Fas/FasL was detected by immunofluoresence
technique.
Result: Expression of Fas on
T cells in 21 cases of the stage of attack (5.81±3.29) and in 15
cases of remission stage (9.86±4.54) were remarkably lower
than those in 25 health children (13.72±5.33) (p<0.01). Expression
of FasL on T cells in the stage of attack (3.22±1.74) was
significantly increased compared with normal control group (2.31±1.05) (p<0.05)
but those in remission patients (2.97±1.28) was not significantly
changed. There was a remarkably negative relationship between expression of
Fas and percentages of peripheral blood lymphocyte (r=-0.632, p<0.01).
Conclusion: The apoptosis of
peripheral blood T lymphocytes from the children with asthma both in stage
of attack and remission are decreased. The inhibited apoptosis might be one
of reasons for the chronic persistent inflammation in the airways in
asthmatic children and suggests that it is necessary to continue therapy in
remission stage.
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