0583

ASSOCIATION OF MUTATION OF Β ₃-ADRENERGIC RECEPTOR GENE WITH OBESITY AND RESPONSE TO DIETARY INTERVENTION OF OBESITY IN CHINESE CHILDREN

Wang Xinli, Tong Xiaomei, Piao Meihua, Li Song

Department of Pediatrics, The Third Hospital Beijing University, Beijing, China

Objective：To determine if a mutation in β₃-abreneric receptor gene was related to children with obesity, dyslipidemia and hyperinsulinemia, and to analyze association of this mutation with response to diet intervention of obesity.

Methods: 311 healthy children aged 8-11 years including 73 overweight and 243 non-overweight were selected randomly from 4 primary schools in Beijing. A mutation of β₃-abreneric receptor gene resulting in the replacement of tryptophan by arginine at position 64 (Trp64Arg) encoding amino acid residues was detected by using the PCR - RFLP for all of them. Fasting insulin and lipids were measured and Anthropometry was carried out. 47 obesity children were selected randomly from overweight children and were separated into two groups. One was diet intervention group (36 subjects); the other was control group (11 subjects). After 3 months of diet intervention, the anthropometry wasd carried out in 47 obesity children again.

Results: Frequency of mutated allele was similar in overweight and normal children (0.18 and 0.17, respectively). Moreover, adjusted for age and gender, there was no significant difference in body mess index (BMI) and the levels of fasting lipids and insulin between those with and without mutation among 311 children. However, after 3 months of diet intervention, the increases of weight and BMI were had significantly lower in obesity children without mutation than in control group (2.41±0.56 vs 4.43±0.70 p<0.05; 0.48±0.24 vs 1.55±0.35 p<0.05, respectively), but the changes in weight and BMI in obesity children with mutation were close to the results in the control (4.32±0.69 vs 4.43±0.70; 1.47±0.32 vs 1.55±0.35).

Conclusions: The Trp64Arg mutation of β₃-abr gene may contribute to the onset of obesity and forecast the result of overweight children with diet intervention to some extent, although the Trp64Arg mutation of β₃-abr gene may not be a major factor effecting on weight in Chinese children.