ANALYSIS OF FUSED GENES RESULTING FROM 29 CHROMOSOME STRUCTURAL ABERRATIONS IN LEUKEMIAS

LI ZG¹, LI P¹, WU MY¹, ZHU P², HU YM¹

¹ Beijing Children’s Hospital, Beijing, China

² The First Hospital of Peking University, Beijing, China

Objective: To explore the diagnostic potential of a novel multiplex nested RT-PCR reaction by screening 29 chromosome structural aberrations in 70 cases with different types of children leukemia/lymphoma.

Methods: A novel multiplex nested RT-PCR reaction was established firstly, which could at the same time screen 29 chromosome structural aberrations/translocations in leukemia/lymphoma with significance to diagnosis and prognosis, then we utilized this method to analyze 70 cases with different types of children leukemia/lymphoma including 54 cases of ALL (Acute Lymphoblastic Leukemia), 9 cases of AML (Acute Myelogenous Leukemia), 4 cases of CML (Chronic Myelogenous Leukemia) including a patient who suffered from NHL (Non-Hodgkin’s Lymphoma) 7 years ago, 3 cases of lymphoma.

Results: 35/70 cases with children leukemia/lymphoma carried 7 types of chromosome aberrations or gene anomalies including t (1;19) (q23;p13), t(8;21) (q22; q22), t (9;22) (q34; q11), dupMLL (11q23), t (12; 21) (p13; q22), TAL1D, HOX11 activation. There are 5 cases, 5 cases, 5 cases, 2 cases, 15 cases, 2 cases, 15 cases with 1; 19, 8; 21, 9; 22, 12; 21 translocation, dupMLL, TAL1D, HOX11 activation, respectively. We also found a patient with T-cell lymphoma carried TAL1D which was usually detected in the patients with T-ALL.

Conclusion: This novel multiplex PCR reaction could be used in screening of the chromosome structural aberrations of leukemia/lymphoma patients, and it can be very helpful to the detection of minimal residual leukemic cells by amplification of the fusion genes resulting from those chromosome structural aberrations.

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