QUANTITATIVE ANALYSIS OF ADHESION MOLECULE CD11B SURFACE EXPRESSION ON NEUTROPHILS FROM NEONATES AND ADULTS

Cui YB1, Du LZ2, Tang YM2, Chen FY1

1 Women and Children’s Hospital, Ningbo, China

2 The Children’s Hospital, Medical School of Zhejiang University, Hangzhou, China

 

Objective:  To investigate the normal values of adhesion molecule CD11b surface expression on neutrophils in neonates and the changes of expression level during development.

Methods: The adhesion molecules CD11b antigen expression on term Neutrophils from the cord blood after uncomplicated vagina deliveries (VD, n=25) and elective Caesarean sections (ECS, n=20) and from the venous blood (n=15) of infants were measured by quantitative flow cytometry with unfractionated whole blood method. Normal healthy adults (n=10) were selected as controls.

Results: The mean fluorescence intensity (MFI) of CD11b antigen were similar among three groups of VD, ECS cord blood and adult venous one (all P>0.05).  The venous blood in term neonates showed much higher CD11b MFI than two cord blood samples and also adult group (all P<0.05).  There was no significant difference in positive percentage of CD11b antigen (CD11b+%) on resting neutrophils among four groups (all P>0.05).

Conclusions: Normal or enhanced adhesion function on resting neutrophils in term infants may have been established. Mode of delivery may not influence neutrophil CD11b expression in term neonates.

 

 
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