THE STUDY ON INTRAMYOCARDIAL CALCIUM
OVERLOAD AND APOPTOSIS INDUCED BY COSACKIEVIRUS B3
Hu XF, Wang HW, Lu WW, Dong YS, Cheng PX
Tongji Hospital, Tongji Medical College, Huazhong
University of Science and Technology, Wuhan, China
Objective: The isolated
cardiac myocytes of rat were immediately affected by cosackievirus B3
(CVB3), in order to research the effect of such procedure on the cell
cycle, apoptosis and intracellular ionized calcium (Ca2+ i) of cardiac
myocytes.
Methods: Newborn
Balb/c murine cardiac myocytes were cultivated, then infected by CVB3.
Fluo-3 is a fluorescent-based calcium-sensitive probe. Labeled
intracellular Ca2+ i with Fluo-3, which was measured by flow cytometer. The
calcium in the cardiac myocytes culture liquid was detected with atom
absorb spectrum test.
Results: CVB3
markedly inhibited the differentiation and proliferation of the infected
cardiac myocytes. It also induced the apoptosis. The intracellular Ca2+ i
level in infected group was higher than that in control group significantly
(P<0.01). The calcium concentration of cardiac myocytes culture liquid
in infected group was lower than that in control group significantly
(P<0.05).
Conclusion: The study
suggested that the apoptosis and intracellular calcium overload of the
CVB3-affected cardiac myocytes are likely to play an important role in the
pathogenesis of viral myocarditis.