EXPERIMENTAL
STUDY ON SERUM NSE AND MBP LEVELS IN RABBITS WITH BILIRUBIN ENCEPHALOPATHY
Li XJ, Guo L, Li L,
Jiang ZHM, Wen XSH
Prevention,
Treatment and Rehabilitation Center for Child Cerebral Palsy in
Heilongjiang Province, Jiamusi, China
Objective: To investigate the
production of animal model with bilirubin encephalopathy and the markers of
neuro-toxitity of bilirubin.
Methods: Thirty-six newborn rabbits were divided into
control group (N n=6), experimental group A (n=6) and B (n=24, T1, T2). Group
A and B were administered bilirubin intraperitoneally in different doses.
The changes of neuro-behaviour and pathology of brain tissue were observed
after 6 and 16 hours and the serum bilirubin, neuro-specific enolase (NSE)
and myelin-basic protein (MBP) levels and bilirubin levels in brain tissues
were determined.
Results: In experimental group B, there were
significant changes of neuro-behaviour and abnomalities of neuron、myelin and Nissle body in different extent according to the doses and
time of bilirubin. The serum bilirubin and MBP levels in group B were
significantly higher than those in group N. The larger the doses and the
longer the time of bilirubin were, the much higher the serum bilirubin and
MBP levels were. The serum NSE levels and bilirubin concentration in brain
tissues significantly increased in the group that were administered
bilirubin after 16 hours, and those in group T2 were higher than those in
group T1. There were positive correlation between bilirubin concentration
in brain tissues and serum NSE、MBP
(r=0.813 r=0.842 p<0.01) and between serum NSE and MBP (r=0.803
p<0.01).
Conclusion: The
animal model with bilirubin encephalopathy can be made successfully by
administration bilirubin intraperitoneally. The serum NSE and MBP levles
are helpful in detecting the damage of neuron and myelin in bilirubin
encephalopathy. They may be used as neuron and myelin markers.