文本框: SETTING IN SITU POLYMERASE CHAIN REACTION IN PERIPHERAL BLOOD CELL AND ITS APPLICATION IN CLINIC Jing C-X, Xie X-Z, Lan D First Affiliated Hospital of Guangxi Medical University, Nanning, China Objective: To set the technique of In Situ Polymerase Chain Reaction (In Situ PCR) in peripheral blood cell in interkinesis and used it to detect heredity disease. Methods: With dig-II –dUTP labeled the primers which amplified the 48 and 52 exons of the gene of dystrophin and complementarity determining region III of IgH (IgH-CDR-III), using In Situ PCR in cell smear and cell suspension to detect Duchenne Muscular Dystrophy (DMD) and III-IV malignant lymphoma. Results: The results showed that there was no signal spot in DMD patients, while the cell in interkinesis of 96% normal male showed signal spots. Among 15 cases newly diagnosed cases with malignant lymphoma, 12 cases can be detected the gene rearrangement of IgH, 4 cases with lymphomatoid leukemia remitted completely after treatment. 2 cases who showed rearrangement of IgH detected by In Situ PCR, recrudesced six months late. Among 22 cases with lymphocytic leukemia, 18 can be detected the rearrangement of IgH. 3 cases remitted completely, but showed positive by In Situ PCR, recrudesced after three months. Conclusion: In Situ PCR did not destroy the cell structure and form, it can fix target cell. The technique combined the advantage of PCR and In Situ hybridization. It can be used to detect acute leukemia and malignant lymphoma, help to predict the recrudescence of the diseases, detect DMD and apply for its prenatal diagnosis. 0733