ANALYSIS OF ANTIGENIC AND GENETIC
VARIABILITY OF RESPIRATORY SYNCITIAL VIRUS
Kong XH, Liu ChY, Wang ShX, Jiang ZF
Beijing Children’s Hospital,
Beijing, China
Objective: To analysis antigenic and
genetic variability of respiratory cyncitial virus (RSV) in Beijing.
Methods: Each
of 20 RSV isolates was reacted with 8 anti-NP, or anti-G antibodies
respectively, and then typed into subgroup A or B by IIF. These isolates
were classified into patterns NP and lineages SHL by restriction mapping of
N gene fragments and sequencing of SH gene. cDNA of the entire G protein
gene of one subgroup A isolate was also sequenced.
Results: Compared with reference isolates, 2
subgroup A isolates from 1990 to 1991 were unreactive with anti-NP antibody
B90 (epitype NP1), 1 subgroup B isolate reacted with B90. Whereas 2
subgroup A isolates from 1997 to 1998 failed to react with anti-NP antibody
C797 (epitype NP1), anti-NP6 antibody B63 (epitype NP6), and anti-G2-G6
antibody B25 (epitype G2-G6), 2 subgroup B isolates were positive with B90
and anti-G2-G6 antibody B14 (epitype G2-G6).
Of 10 isolates from 1990 to 1991, 8 subgroup A isolates gave pattern NP3
(SHL2), 2 subgroup B isolates NP4. With 10 isolates from 1997 to 1998, 2
subgroup A isolates fell into NP3 (SHL2), 8 subgroup B NP4, but the ratio
of subgroup A to B was reverse (4:1 v1:4).
Comparison of the sequences of the G
genes of one subgroup A isolate in 1998 and prototype A2 showed that there
was 6.7% nucleotide sequence variability and 11.4% amino acid sequence
variability overall. Moreover, the variability was concentrated in the
ectodomain of the G protein.
Conclusion There was extensively antigenic and genetic variability between
subgroup A and B, even within subgroups.
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