文本框: THE STUDY ON THE TREATMENT OF EXPERIMENTAL AUTOIMMUNE NEURITIS BY ORAL MYELIN BASIS PROTEIN Liang D, Jia F-Y, Li H-B, Chen Y-B, Li C-H,Feng S-F The Frist Hospital of JiLin University, Changchun, China Objective: To interprete the treatmental effect of bovine pheripheral myelin (BPM) inducing the immunetolerance of experimental autoimmune neuritis (EAN). Methods: Guinea pigs EAN model was established by BPM and was divided into natural course group, oral BPM group, IVIG intraperitoneal group and normal control group. EAN guinea pigs were assesed by international grade and clinical scores standard. T cell subsets of sciatic nerve tissue were detected by immunocytochemistry; the proliferation change of spleen lymphocyte was determined by MTT; routine pathology, semi-thin dissection and the demyelination and the remyelination of sciatic nerve were systematically assesed. Results: The EAN ratio induced by BPM was 87.5%, and the onset of typical paralysis was following inducing from 12 to 16 days. The proliferation assay showed: the significant proliferation occured at the 7th day after the first immunization, reached its peak at the 14th day, decrased mildly after the 21st day. No significant proliferation of spleen lymphocyte to BPM was found at the 21th and the 28 th day in oral BPM group. The pathologic change of sciatic nerve were infiltrated predominantly by CD4+T cell. The nerve fiber present swelling, dissecting, demyelination. In the BPM group and the IVIG group, not only the clinical symptom but also neuropathological change restored significantly and showed decreasing demyelination and increasing remyelination at 21st day. Conclusion: Oral BPM treatment can amelioate the EAN and lessen the sever clinical symptoms, and the mechanism may be that high -dose oral BPM inducing immune tolerance by the deletion or anergy of autoreactive CD4+T cell, and attain the whole immune modulating effect. 0781