THE
EXPRESSION AND IMPLICATIONS OF R¨CIFN, NO IN MURINE EXPERIMENTAL VIRAL
MYOCARDITIS
Cheng Yan, Yang
Zhengxiu,
Department of
Pediatrics,
Affilated hospital of
Anhui Medical University, Hefei, China
Objective: To
investigate the effect of r-IFN, NO expressed in experimental viral
myocarditis.
Methods: 60 BALB/C
mice were randomly divided into 4 different groups. 15 mice were used as
control group, 45 of them were intraperitoheally injected with CVB 3m and
then divided into 3 groups (B,C,D). 3 days later mice in C, D groups were
injected MP (methyprednisolone) intraperitoheally (30mg/kg, 60mg/kg
respectively and daily for 3days). Blood and heart samples were obtained
from all 4 groups every 7 days (5 mouse per group per time) for measurement
of r-IFN, NO and pathology.
Results: (1) The
level of r-IFN, NO were general raised in viral group (B), (2) MP can
reduce the production of r-IFN, NO. The more the dose given, the lower they
expressed. The survival in D
group is the lowest controlled with B or C. The pathology of C is better
than the other 2 groups.
Conclusion: r-IFN and NO
play an important role in the pathogeneses of virus induced myocarditis.
Both overproduction and decreasing excessively may do harm to antiviral
process. The proper use of cotisol may amilated the cytokine level and
benefit to the animal¡¯s recovery.