TRANSFER
OF ANTISENSE-VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) INHIBITING
NEUROBLASTOMA GROWTH IN VIVO
Cao WG, Zhang JZ
Beijing Children's
Hospital, Beijing, China
Objective: To
investigate the effectiveness of antisense-VEGF121 on the human neuroblastoma.
Methods: Sixteen nude
mine, female, 4-6 weeks of age, were divided into two groups randomly. After neuroblastoma SH-SY-5Y cells
were injected into the flank of the nude mouse, intratumoral treatment with
brine medium of either pcDNA3.1-antiVEGF or pcDNA3.1 was started and
repeated two times per week for a total of six times. The largest and
smallest diameters of each tumor were recorded for measuring tumor volume
before each injection. At the end of the measurement period, the mice were
sacrificed by CO2, their tumor were excised and fixed in neutral-buffered
formalin for H&E for normal control and immunohistochemistry staining
for detecting VEGF and Ⅷ related antigen.
Results: The volume
of tumor in the groups treated with pcDNA3.1-antiVEGF was smaller
significantly than that of control treated with pcDNA3.1 (reduced by
78.5%.P<0.001),and the expression of VEGF and Ⅷ related antigen was lower in treating group
than in control(P<0.01).
Conclusions: Antisense-VEGF gene transfer
mediated by pcDNA3.1 can inhibit the growth of tumors derived from the
human neuroblastoma SH-SY-5Y cells.