TYPE I INSULIN-LIKE
GROWTH FACTOR RECEPTOR AND CORD BLOOD LYMPHOCYTE FUNCTIONS
Yang Y, Niu JY, Guo LZ
Children¡¯s Hospital of Fudan University,
Shanghai, China
Objective: To study effects of
expression of mRNA for type I insulin-like growth factor receptor (IGF-IR)
on human cord blood lymphocytes (CBL).
Methods: Using reverse
transcription-polymerase chain reaction (RT-PCR) technique, we observed the
expression of IGF-IR at mRNA level in CBL. The antisense oligonucleotide
was used to inhibit the expression of IGF-IR gene of CBL. We measured IL-2,
4, IFNg mRNA levels
by RT-PCR, immunoglobulin M (IgM) production by CBL with ELISA, and
lymphocyte proliferation by 3H thymidine uptake technique.
Results: IGF-IR mRNA was
detected in both rest and activated CBL, and the expression level in the
activated CBL was higher than the rest. After exposure to antisense
oligonucleotide a 50% reduction in IGF-IR mRNA was observed. In addition,
the proliferation of PHA stimulated CBL was significantly reduced about
50%, the production of IgM from the CBL was markedly decreased, the mRNA
levels of IFNg and IL-2
decreased by 30% ~ 50%, and IL-4 decreased 20% ~ 30%.
Conclusion: IGF-IR involved in the
process of CBL proliferation and production of immunoglobulin and
cytokines. It might play an important role in the modulation of the immune
functions.