文本框: ANOXIA OF NEWBORN AND RENAL INJURE Liu L, Sao XF Third Center Hospital, Tianjin, China Objective: Neonatal kidney was agenesis and produced easily renal dysfunction when some pathologic factor affected it. To early discover renal dysfunction had important significance to be directed in clinical diagnosis and treatment. The microproteinuria of 106 neonates was tested and to study damage degree of the glomerulus and renal tubules in neonates with anoxia of different period and degree. Methods: The uric retinal–binding–protein (RBP) and N-acctyl-B-D-Glucosaminidase (NAG) and microalbuminuria (MA) were measured in 24 neonates with intrauterine distress, 10 neonates with childbirth asphyxia, 24 neonates with postpartum anoxia and 48 healthy neonates (control group). RBP was tested by enzyme linked immunosrbent assay (ELISA). NAG used speed method and assayed the increment of substrate absorptance and computed the contents of NAG. MA was assayed by immunoturbidimetry. Statistical method: The microprofeinuria was showed by ratio of protein and uric creatinine. To use the t-test and analysis of variance. The dates were showed by X+S. Results: The levels of RBP, NAG, MA were increased in intrauterine distress, childbirth asphyxia and postpartum anoxia than control group and 4 group each other were significantly different. The changes of RBP and NAG were correlated with the contaminate degree of amniotic fluid and showed different degree anoxia. The amniotic fluid Ⅲ degree were significantly differences than Ⅱ degree in uric RBP and NAG ,but MA was no significant differences. The uric RBP, NAG, MA of neonates with intrauterine distress and childbirth asphyxia and postpartum with hypoxic-ischemic encephalogathy (HIE) were most important increased. See Table 1. and Teble 2. Conclusion: The RBP, NAG, MA levels of urine showed different changes when the anoxia was different period and degree. The RBP, NAG, MA levels were correlated with the severity and long time of anoxia. So the microproteinuria could show early damage of kidney and prompt us to pay attention to treating injure of kidney when we treated brain injure. Table 1. The uric RBP, NAG, MA changed in different period anoxia Group n RBP (µg.mmol-1Cr) NAG(µ.g-1Cr) MA(mg.mmol-1Cr) Control group 48 202.46+142.84 31.99+14.98 18.09+7.20 Intrauterine distress 24 875.16+560.30 189.71+125.71 51.14+26.22 Childbirth asphyxia 10 869.50+416.15 185.77+132.75 58.11+22.22 Postpartum anoxia 24 274.53+234.63 94.46+59.47 40.23+24.0 F 11.424 9.923 8.113 Note: P<0.01 Table 2. The uric RBP, NAG, MA changed in different degree with the contaminate amniotic fluid Group n RBP(µg.mmol-1Cr) NAG(μ.g-1Cr) MA(mg.mmol-1Cr) Amniotic fluid Ⅱdegree 10 209.55+124.48 61.39+28.53 34.66+18.87 Amniotic fluid Ⅲ degree 9 908.98+534.05 161.67+63.34 44.21+27.02 t 5.637 * 5.441 * 1.410 Note: * P<0.05, MA P>0.05 0871