DETECTION OF HOSPITAL INFECTION BY RANDOMLY AMPLIFIED POLYMORPHISM DNA

Sun LW, Bao CT, Da SE

Changchun Childrens Hospital, Changchun, China

 

Objective: Changchun Childrens Hospital has 506 beds. 87 percent of them are eight-person rooms. Cross infection in hospital is a very prominent problem. Our aim is to inquire into the source of infection and trace the route of transmission so that we can set up the method that monitor the cross infection in hospital exactly and quickly.

Methods: random select two primers from deca-polymorphism DNA oligonucleotides and Alu 278 primers by using the method designed by the opreon Company in the U.S. Through expand to staphylococcus aurous (SA), Shivas bacillus, Escherichia coil, salmonella enteritis,  Pseudomonas aeruginous (PA), candida albicans, reparatory syncytial virus (RSV) and adenovirus we can determine the cross infection in our hospital.

Result: Spending zone of 39 specimens from respiratory ward in February 1998 is the same with that of 13 specimens from digestive ward in 2000. 39 specimen of them have separated RSV, and has been identified by neutralization test. 13 specimen from digestive ward are appraised as rotavirus by Chinese Academy of Preventive Medicine. We monitored the cross infection in hospital of two PA and one SA. The rate of cross infection in hospital in 2000 has been cut down by 1.14% than that of the last year, which has saved 699720 Yuan for patients, and has earned 265360 Yuan for our hospital.

Conclusion: Technology of random polymorphic DNA cloning can both determine the source of infection promptly in molecular level and trace the route of transmission.

 
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