文本框: THE EFFECTS OF HUMAN INTERLEUKIN-4 GENE TRANSFER ON DIFFERENTIATION AND APOPTOSIS IN HEPATOBLASTOMA CELLS Fu JR1 , Li CR1, Zhou YD2 1Children’s Hospital of Shenzhen, Shenzhen, China 2 Children’s Hospital of Chongqing, Chongqing, China Objective: To investigate the effects of human interleukin-4 (IL-4) gene transfer on differentiation and apoptosis in hepatoblastoma cells line (HepG2) cells. Methods: Retroviral vector was employed to introduce human IL-4 gene into hepatoblastoma cell line (HepG2) cells. Trypan blue exclusion, whight dye, radioimmunoassay ,in situ hybridization ,Polymerase chain reaction-Enzyme linked immunoadsordent assay (PCR-ELISA) were used to determine its change in morphology and cell cycle . The apoptosis and the expression of p53, bcl-2 were analyzed by flow cytometry. Results: The proliferation of the cells transfered by human IL-4 gene were surpressed (p<0.05) and its shape tended to become relatively mature while the expression of protooncogenes such as c-fos ,c-jun,c-myc were inhibited (p<0.001) ; Human IL-4 gene transfer could also induce the apoptosis in HepG2 cells, the ratio of apoptosis added up to 11.3±2.75% (p<0.01) at the same time the expression of P53 and bcl-2 were increased and decreased respectively. Conclusion: Human IL-4 gene transfer could induce the apoptosis and differentiation in hepatoblastoma cells, the occurrence of apoptosis was probably related to up-reglated the expression of P53 and down-reglate the expression of bcl-2. 0911