文本框: A NEW NON-INVASIVE TEST FOR DETECTION OF HELICOBACTER PYLORI INFECTION IN PEDIATRIC PATIENTS — HELICOBACTER PYLORI STOOL ANTIGEN ASSAY Kang H-Z, Li B-B, Chen F-H Ninth People’s Hospital, Shanghai Second Medical University, Shanghai, China Objective: To evaluate the reliability of the Helicobecter Pylori (Hp) stool antigen (HPSA) for detection of Hp infection in pediatric patients. Method: Stool specimens were collected from 68 pediatric patients who have received gastroendoscopy examination and from 13 child patients who have received 13C-Urea Breath Test (13C-UBT). Total 81 specimens were collected from pediatric patients aged ranging from 4 to 14 years old. The evaluation of Hp status was based on culture and direct microscopic examination of a Gram staining smear and/or 13C-UBT detected. A commercial kit by enzyme-linked immunosorbent assay was used to examine the HPSA in the stool. Hp status was defined as positive when the culture and/or direct microscopic examination of the smear were positive and/or 13C-UBT detect result over 3.5‰. Result: The pediatric patients with symptom of digestive tract before the eradication of Hp showed that 48 of 81 cases were diagnosed as Hp positive and 33 cases as negative. The HPSA test was positive in 48 cases and negative in 33 cases. The 68 cases were received biopsy. 43 pediatric patients were positive. The HPSA test was positive in 40 cases and negative in 3 cases. 25 pediatric patients were negative. The HPSA test showed negative in 23 cases and positive in 2 cases. The sensitivity and specificity were 93.02% (40/43) and 92% (23/25) respectively. The accuracy rate was 92.65% (63/68). 7 of 68 cases were received 13C-UBT detect at the same time. All of the cases showed positive. The HPSA test was positive, too. The 13 cases with symptoms of digestive tract were received 13C-UBT detect. 6 of the 13 cases showed positive and their HPSA test also showed positive. 7 cases of the 13 showed negative and their HPSA test showed negative too. The sensitivity, specificity and accuracy rate all were100%(13/13 .7/7. 20/20). Conclusion: The HPSA test is a new, simple, non-invasive method for accurate diagnosis of current Hp infection. This method has particularly high accuracy rate in pediatric patients before anti-Hp therapy. And it is particularly valuable for the popularization of its clinical use. 0912