文本框: A STUDY ON MIXED CORD BLOOD TRANSPLANTATION BY ANIMAL TEST
Ju X-L, Shen B-J, Hou H-SH, Shi Q
Qilu Hospital, Shandong University, Jinan, China

Objective: To investigate the potential possibility to reconstitute the immunohematopietic system in adult mice recipients across major histocompatibility complex by mixed near-term cord blood transplantation and to lay a foundation for the study of mixed cord blood transplantation.
Methods: To inject 1×106 fetal blood cells from 20-day-old C57BL/6 (H-2b) pups and 1×106 fetal blood cells from 20-day-old 615(H-2k) pups delivered by cesarean into a lethally irradiated(8.5Gy) BALB/c(H-2d) adult mouse in the same time. After one month, anti-H-2Db and anti-H-2Kk antibody was used to investigate donor chimerism in Recipients. In vitro mixed lymphocyte response (MLR) and skin transplantation were used to investigate specific tolerance.
Results: All of the control mice receiving lethal radiation died within three weeks after radiation. 70% recipients survived without any clinical signs of graft versus host disease (GVHD) within the observation period of three months. Two donors’ mixed chimera was demonstrated by using anti-H-2Db and antu-H-2Kk antibody in the same recipient. The inability to reject the two donors’ skin indicated immune tolerance was established in the recipients. Immune tolerance was also confirmed by in vitro mixed lymphocyte response.
Conclusions: Two kinds of fetal blood can construct mixed chimera in the same adult mice recipients. Mixed fetal blood transplantation can reconstitute the immunohematopietic system in adult mice recipients across major histocompatibility complex without causing GVHD.
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