DETECTION OF HPVB19, RV GENE
IN CARDIAC TISSUE FROM CHILDREN WITH CONGENITAL HEART DISEASE
Zhang G-C, Wang X-M, Xu D-L, Cai H, Sun X, Li B.
Xijing Hospital, Fourth
Military Medical University, Xi’an, China
Objective: Congenital heart disease
(CHD) is the most popular malformation of human birth defects. The
incidence of CHD is around 0.5~1.0% in live newborn and 10% in natural
abortion. The pathogeny of CHD is not known yet. The main cause of CHD is
regarded to be the interaction of inherent susceptibility and enviromental
malformation factor, which mostly refers to intra-uterine virus infection.
This article is to explore relationship of HPVB19, Rubella virus(RV) and
other pathogeny infection with CHD.
Methods: HPVB19,Toxoplasma (TOX),
RV, Herpes simplex virus (HSV) and other pathogenic gene were detected by
PCR, RT-PCR and B19 viral location was studied in cardiac tissues by ISH in
79 cases of CHD and 46 cases without CHD of children.
Results: (1) The positive rate of B19, Tox, RV and
HSV gene in cardiac tissue of CHD was 18.2% (12/66), 12.7% (10/79), 23.3%
(7/30) and 3.8% (3/79) respectively. The positive rate of B19, TOX and RV
was significantly different (P<0.05) between CHD and control group,
while no statistical different (P>0.05) for HSV. The results show that
B19, TOX and RV infection might be important risk factors for CHD. (2)
Specific IgM of B19, TOX and RV is negative from serums of the patients in
biopsy group whose gene detection is positive at the same time. (3) B19 DNA
is located the nucleus of cardiac cell which has no inflammation under
optical microscope.
Conclusions: As far as we know, it
is the first report of the presence of HPVB19, TOX and RV in cardiac
tissues of CHD patients. The detected rates are significantly different
between the two groups, inferring that they might be correlated with CHD.